Genome Analysis Technology Core

 To continue to receive funding from the Cancer Center and School Of Medicine, we are encouraged to demonstrate the core's impact on scientific output in the form of a citation in publications and/or presentations generated using the core services.  PLease let us know what services were used by include the core name and core ID  on your slides and peered review publications  eg.: This work was supported by the UVA Genome Analysis and Technology Core, RRID:SCR_018883.  When  appropriate thank the core staff by name or include them as co-Authors.  We routinely provide Method sections write up and proof reading.  

Mission Statement

The GATC continuing mission is to serve as a scientific resource and biotechnology hub enhancing the scope and quality of basic and translational research at UVA, and to provide customer satisfaction, collaboration and quality control.The goal of the core staff is to enable high throughput (Next Generation) sequencing projects for both novice and experienced users alike, by providing timely assistance in all facets of experimentation from project design/execution to data analysis/reporting. 

Brief Overview of Services

10x Genomics library prep services

• GEM-X - 5' and 3' RNA seq Library prep
• GEM-X VDJ - T or B cell with 5' RNAseq
• ATACseq and MultiOmics (ATAC-seq and GEX)
• Cell-Plex and Feature barcode with 3' or 5' RNAseq
• Fixed Cell (probe based)

Illumina Single cell Library prep- PIP-Seq (under development)

• T2 prep: 5K input cells and 100M reads per sample.
• T10 prep: 17K input cells and 340M reads per sample.
• T20 prep: 40K input cells and 800M reads per sample.
• T100 prep:200K input cells and 4B reads per sample.

NEW PAcBio Long Read sequencing on the REVIO (under development)

• Genomic DNA
• Bulk RNA seq
• Single cell RNA seq

Illumina NextSeq2000 sequencer (NSQ2K)

• Flow cell read output: 100 Million (P1), 400 Million (P2),  1.2 Billion (P3), and 1.8  Billion (P4) reads per run
• Sequencing read lengths: 50 bp (2X25), 100bp (2X50), 200bp (2X100), and 300bp (2X150)

Illumina MiSeq

• QC runs Nano 1.2 millions or  Micro 3 million reads 300 cycle kits
• V2 (12-14 Million reads) and V3 (24million reads) chemistries offered

Library Prep for Illumina's NextSeq and MiSeq

Leadership

Location and hours of operation

Links and Resources

UVA RESEARCH CORES HOME PAGE

GATC Home Page